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Janus-structured GOx-Fe/cu Nanozymes for broad-range glucose detection and Milk analysis
Jiajie Xu1, Yuhang Huang2, Jiayu Xu2
1State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.
A novel Janus-structured glucose oxidase-iron/copper nanozyme enhances stability and detection range for glucose biosensing. This breakthrough enables portable, visual glucose quantification in food samples using a smartphone.
Area of Science:
- Biomaterials Science
- Analytical Chemistry
- Nanotechnology
Background:
- Glucose oxidase (GOx) instability under varying temperature and pH limits biosensor applications.
- Current nanozyme glucose sensors exhibit narrow detection ranges and poor stability.
Purpose of the Study:
- To develop a stable and highly sensitive nanozyme for glucose biosensing.
- To broaden the detection range and improve the stability of glucose sensors.
- To create a portable detection platform for glucose and hydrogen peroxide in food samples.
Main Methods:
- One-pot self-assembly synthesis of Janus-structured GOx-Fe/Cu nanozyme using Fe₁₋ₓS and GOx on Cu₃(PO₄)₂ nanoflowers.
- Integration of the nanozyme into a chitosan/agarose hydrogel for visual quantification.
- Smartphone-based portable detection system development.
- Validation in milk samples for glucose and hydrogen peroxide detection.
Main Results:
- Achieved an ultra-broad glucose detection range (0.5–20 mM) with a low limit of detection (34.18 μM).
- Demonstrated enhanced stability: >80% activity after 7 hours at pH 3 and 5 hours at 60 °C.
- Showcased high specificity and reliable detection in milk samples (95–121% recovery, 0.31%–8.26% RSD).
Conclusions:
- The Janus-structured GOx-Fe/Cu nanozyme significantly improves GOx stability and sensing performance.
- The hydrogel platform facilitates visual and portable detection of glucose and hydrogen peroxide.
- The developed system shows practical applicability in food chemistry analysis.
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