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Janus-structured GOx-Fe/cu Nanozymes for broad-range glucose detection and Milk analysis
Jiajie Xu1, Yuhang Huang2, Jiayu Xu2
1State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.
Abstract:
Glucose oxidase (GOx) is unstable under temperature/pH variations, limiting its biosensing applications. Existing nanozyme-based glucose sensors suffer from narrow detection ranges (0.2-12.0 mM) and poor stability. Herin a Janus-structured GOx-Fe/Cu nanozyme was synthesized via one-pot self-assembly with Fe₁₋ₓS and GOx partially-covered on Cu₃(PO₄)₂ nanoflowers. This nanozyme achieved an ultra-broad glucose detection range (0.5-20 mM, LOD = 34.18 μM), 40 times wider than existing nanozyme, with enhanced stability (>80% activity after 7 h at pH 3 and 5 h at 60 °C) and high specificity. When integrated into a chitosan/agarose hydrogel, it enabled visual glucose quantification through smartphone-based portable detection. In milk samples, it showed reliable glucose and H₂O₂ detection with recovery rates of 95-121% and relative standard deviations (RSD) of 0.31%-8.26%, demonstrating practical applicability. This Janus-structured GOx-Fe/Cu nanozyme improves GOx stability and sensing performance, and the hydrogel platform enables portable detection of glucose and hydrogen peroxide in food chemistry.
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