Lactobacillus reuteri DSM 17938 Improves Intestinal Motility in Mice With Postoperative Ileus by Modulating Gut
Jingyi Tang1,2, Yingnan Hu1,3, Dian Yu1
1The Second Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, China.
Background:
Postoperative ileus (POI) is a common complication after abdominal operation, which is characterized by delayed gastrointestinal motility that can lead to prolonged hospitalization. Previous studies have demonstrated that gut microbiota and macrophages are involved in POI pathogenesis, but the underlying mechanism and the role of probiotics in it are still unclear. This work investigated the prophylactic effect of Lactobacillus reuteri (L. reuteri) DSM 17938 administration on POI mice.
Methods:
Mice were supplemented with antibiotics or L. reuteri DSM 17938 for two weeks before intestinal manipulation surgery. One day after surgery, the small intestine transit rate was evaluated. Samples were collected to determine the intestinal macrophages, histology, cytokines, gut microbiota, and fecal metabolome.
Key Result:
Results showed that gut microbiota alteration contributed to POI. L. reuteri DSM 17938 increased intestinal motility of POI mice, maintained the proportion of intestinal CX3CR1+ macrophage, reduced M1 macrophage, and modulated macrophage-associated cytokines. Additionally, L. reuteri DSM 17938 slightly alleviated gut metabolic disorders but had an effect on a portion of metabolites of fecal tryptophan metabolism, modulated gut microbiota composition and function, and specifically suppressed bacterial invasion of epithelial cells and the expansion of Escherichia coli, which had a negative correlation with the small intestine transit rate in POI mice.
Conclusions And Inferences:
Our findings indicate that L. reuteri DSM 17938 is a promising probiotic for the prevention of POI, but it still needs further clinical validation.
Insights
Lactobacillus reuteri (L. reuteri) DSM 17938 probiotic may prevent postoperative ileus (POI) by improving gut motility and modulating gut microbiota. Further clinical studies are needed to confirm its efficacy in patients.
Area of Science:
- Gastroenterology
- Microbiology
- Immunology
Background:
- Postoperative ileus (POI) is a common complication following abdominal surgery, characterized by delayed gastrointestinal motility and prolonged hospitalization.
- The roles of gut microbiota and macrophages in POI pathogenesis are recognized, yet underlying mechanisms and probiotic efficacy remain unclear.
Purpose of the Study:
- To investigate the prophylactic effects of Lactobacillus reuteri (L. reuteri) DSM 17938 on postoperative ileus in a mouse model.
Main Methods:
- Mice received antibiotic or L. reuteri DSM 17938 supplementation for two weeks prior to intestinal manipulation surgery.
- Post-surgery assessments included small intestine transit rate, intestinal macrophages, histology, cytokines, gut microbiota, and fecal metabolome analysis.
Main Results:
- L. reuteri DSM 17938 enhanced intestinal motility in POI mice, maintained CX3CR1+ macrophage proportion, reduced M1 macrophages, and modulated related cytokines.
- The probiotic also alleviated gut metabolic disorders, influenced tryptophan metabolism, modulated gut microbiota, and suppressed Escherichia coli expansion, which correlated negatively with intestinal transit.
Conclusions:
- L. reuteri DSM 17938 demonstrates potential as a probiotic for preventing POI.
- Further clinical validation is required to confirm its therapeutic benefits in human patients.
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