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Organelle Localization-Induced Bio-Orthogonal Polymerization (OLIBOP) for Photostable Super-Resolution Live-Cell

Gaeun Park1,2, Sangpil Kim1, Dohyun Kim1

  • 1Department of Chemistry, College of Natural Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.

Advanced Healthcare Materials
|February 11, 2026
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Summary
This summary is machine-generated.

Researchers developed a new method for creating photostable fluorescent probes within specific cell parts, enabling long-term live-cell imaging of mitochondrial dynamics without photobleaching.

Keywords:
aggregation‐induced emissionbio‐orthogonal reactionin situ polymerizationlive‐cell imagingorganelle

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Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Cell Biology

Background:

  • Photobleaching limits long-term live-cell imaging of dynamic biological processes.
  • Polymeric Aggregation-Induced Emission luminogens (AIEgens) offer photostability but often localize to lysosomes.
  • There is a need for probes with broader subcellular accessibility and improved photostability.

Purpose of the Study:

  • To develop a novel approach for synthesizing photostable polymeric fluorescent probes in situ within specific organelles.
  • To overcome the limitations of current probes, particularly lysosomal localization and photobleaching.
  • To enable real-time, long-term imaging of cellular dynamics, specifically mitochondrial functions.

Main Methods:

  • Organelle Localization-Induced Bio-orthogonal Polymerization (OLIBOP) strategy was employed.
  • A small-molecule precursor (1-AIE) was designed with AIE properties, mitochondria-targeting moiety, and a bio-orthogonal condensation group.
  • Mitochondrial localization and in situ polymerization were triggered by a GSH-responsive disulfide bond, visualized using phasor-FLIM.

Main Results:

  • The designed precursor (1-AIE) successfully polymerized in situ specifically at mitochondrial sites.
  • The resulting in situ-formed poly-AIEgen exhibited significantly enhanced fluorescence intensity and longer fluorescence lifetime.
  • Exceptional photostability allowed for unprecedented real-time tracking of mitochondrial dynamics over extended periods.

Conclusions:

  • OLIBOP provides a breakthrough approach to overcome the trade-off between probe delivery and stability.
  • This method enables the creation of photostable polymeric probes within target organelles, enhancing biocompatibility.
  • The developed probes facilitate high-resolution, long-term live-cell imaging of cellular processes, particularly mitochondrial dynamics.