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Published on: May 25, 2018
Dual-pronged genome engineering: Enhancing crop resistance through elite allele pyramiding and susceptibility gene
Qingdong Zeng1, Zhensheng Kang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection Northwest A&F University Yangling Shaanxi China.
Abstract:
Developing durable crop disease resistance remains a primary breeding objective, achievable through pyramiding resistance genes and editing susceptibility (S) genes. A recent study identified 10 stable Verticillium wilt resistance genes and eight negative regulators via integrated genome-wide association studies and transcriptome-wide association studies-eQTL analyses in upland cotton. Both pyramiding resistance alleles and CRISPR-mediated knockout of S-genes significantly reduced disease severity. These findings enable rapid implementation of disease resistance improvement in the genomics era.
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