Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ovaries01:26

Ovaries

2.6K
The ovaries are roughly the size of almonds and measure approximately 2 to 3 centimeters in length. These paired structures are situated within the pelvic region and are anchored by the mesovarium—a peritoneal extension that also connects them to the wider structure of the broad ligament. The support system extends to the suspensory ligament, housing blood and lymphatic vessels. In addition, the ovarian ligament tethers the ovaries to the uterus.
On the ovarian surface, a layer of...
2.6K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.7K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.7K
Replicative Cell Senescence02:15

Replicative Cell Senescence

4.4K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.4K
Aging01:26

Aging

801
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
801
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

896
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
896
Stomach Histology01:26

Stomach Histology

3.3K
The stomach comprises several layers that work together to facilitate digestion and protect the organ. The outermost layer is called the serosa, which provides support and protection to the stomach. The muscularis externa layer is responsible for the mechanical breakdown of food by contracting and moving the stomach. The submucosa layer, located beneath the muscularis externa, contains connective tissue, blood vessels, nerves, and glands that secrete mucus and other substances essential for...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial mapping and senolytic targeting of senescent and disease-associated microglia in aged mouse brain white matter.

Nature aging·2026
Same author

CODAvision: best practices and a user-friendly interface for rapid, customizable segmentation of medical images.

Nature protocols·2026
Same author

Immune aging biomarkers for clinical trials.

Nature medicine·2026
Same author

A phenotypic <i>in vitro</i> assay of mouse oocyte maturation identifies reversible blockers of meiotic progression for non-hormonal contraceptive discovery.

Human reproduction open·2026
Same author

Quantitative 3D histology reveals localized immune remodeling during early pancreatic cancer progression.

Cell press blue·2026
Same author

Biocompatible designated Resin-3D-printed polymers exhibit reproductive toxicity prevented by Parylene-C.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Feb 13, 2026

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

805

Senescence-Linked Fibrosis in the Aging Human Ovary Revealed by p16-Based Histological Profiling and Spatial

Birgit Schilling1, Mark Watson1, Pooja Devrukhkar2

  • 1Buck Institute for Research on Aging.

Research Square
|February 12, 2026
PubMed
Summary

Cellular senescence drives ovarian aging. Researchers mapped senescent cells in postmenopausal ovaries, revealing fibro-inflammatory microenvironments that are potential therapeutic targets to preserve ovarian function.

Keywords:
Senescencefibrosismenopauseovarian agingspatial transcriptomics

More Related Videos

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

4.5K
Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
07:40

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Published on: May 16, 2025

985

Related Experiment Videos

Last Updated: Feb 13, 2026

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

805
Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

4.5K
Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
07:40

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Published on: May 16, 2025

985

Area of Science:

  • Reproductive biology
  • Cellular biology
  • Gerontology

Background:

  • Cellular senescence is a key driver of ovarian aging.
  • Senescent cells in the human postmenopausal ovary are not well understood.

Purpose of the Study:

  • To identify and map senescent cells and their microenvironments in the postmenopausal ovary.
  • To characterize the molecular signature and spatial distribution of senescent ovarian cells.

Main Methods:

  • Utilized p16INK4a protein expression, immunohistochemistry, and multiplexed immunofluorescence to locate senescent cells.
  • Employed spatial transcriptomics and AI-guided digital pathology for microenvironment mapping.
  • Analyzed whole-transcriptome profiles and collagen matrix architecture.

Main Results:

  • Identified discrete clusters of senescent cells (p16-positive) in stromal, vascular, and cyst-associated regions, increasing with age.
  • Discovered a 32-gene signature (BuckSenOvary) associated with senescent regions, characterized by suppressed cell-cycle regulators and activated inflammatory/ECM remodeling genes.
  • Confirmed fibro-inflammatory nature of senescent microenvironments with complex collagen matrix.

Conclusions:

  • Senescent ovarian niches are spatially defined, fibro-inflammatory microenvironments.
  • These senescent niches are characterized by a distinct molecular signature (BuckSenOvary).
  • Targeting these senescent ovarian niches may offer a strategy to preserve ovarian function.