Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Molecular Models02:00

Molecular Models

43.9K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
43.9K
Growth Models with Integration: Problem Solving01:27

Growth Models with Integration: Problem Solving

63
In population modeling, integration provides a systematic way to determine accumulated quantities from known rates of change. One such application arises in ecology, where the total weight of a fish population in a body of water is referred to as its biomass. When the rate of growth of this biomass is known as a function of time, calculus can be used to determine the total biomass at a future date.Growth Rate and Biomass FunctionLet the growth rate of the fish population be represented by a...
63
Fixation and Sectioning01:03

Fixation and Sectioning

8.0K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
8.0K
Molecular Orbital Theory II03:51

Molecular Orbital Theory II

27.7K
Molecular Orbital Energy Diagrams
27.7K
Structure of Benzene: Molecular Orbital Model01:18

Structure of Benzene: Molecular Orbital Model

12.8K
According to the molecular orbital (MO) model, benzene has a planar structure with a regular hexagon of six sp2 hybridized carbons. As shown in Figure 1, each carbon is bonded to three other atoms with C–C–C and H–C–C bond angles of 120°. The C–H bond length is 109 pm, and the C–C bond length is 139 pm which is midway between the single bond length of sp3 hybridized carbons (154 pm) and sp2 hybridized carbons (133 pm).
12.8K
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

741
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
741

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ERGA-BGE reference genome of the lineid heteronemertean <i>Lineus lacteus</i> (Pilidiophora, Nemertea).

Open research Europe·2026
Same author

New insights into the <i>Syllis prolifera</i> species complex from the eastern Mediterranean Sea.

ZooKeys·2025
Same author

No Mate, No Problem: Molecular Mechanisms Involved in Parthenogenesis in the Cosmopolitan Earthworm Aporrectodea trapezoides (Annelida, Clitellata).

Molecular ecology·2025
Same author

Integrative taxonomy in <i>Syllis prolifera</i> (Annelida, Syllidae): from a unique cosmopolitan species to a complex of pseudocryptic species.

Invertebrate systematics·2024
Same author

Annelid adult cell type diversity and their pluripotent cellular origins.

Nature communications·2024
Same author

Drivers of species knowledge across the tree of life.

eLife·2023

Related Experiment Video

Updated: Feb 13, 2026

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory
06:00

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory

Published on: July 10, 2018

13.5K

A Field-Friendly, Non-Toxic Fixative for Integrated Morphological and Molecular Research in Non-Model Invertebrates.

Irene Del Olmo1, Paula Moreno-Martín1, Patricia Álvarez-Campos1

  • 1Departamento de Biología Universidad Autónoma de Madrid Madrid Spain.

Ecology and Evolution
|February 12, 2026
PubMed
Summary

KINFix is a novel fixative that preserves both morphology and molecules in non-model invertebrates. This versatile, cost-effective solution is ideal for field research, enabling diverse studies from a single sample.

Keywords:
KINFixRNA integrityRNAlaterSEMfieldworknon‐model organismssample fixationsample preservationsingle‐cell RNA sequencing

More Related Videos

Assaying &#946;-amyloid Toxicity using a Transgenic C. elegans Model
13:59

Assaying β-amyloid Toxicity using a Transgenic C. elegans Model

Published on: October 9, 2010

26.7K
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex
09:23

Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

Published on: June 30, 2019

12.6K

Related Experiment Videos

Last Updated: Feb 13, 2026

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory
06:00

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory

Published on: July 10, 2018

13.5K
Assaying &#946;-amyloid Toxicity using a Transgenic C. elegans Model
13:59

Assaying β-amyloid Toxicity using a Transgenic C. elegans Model

Published on: October 9, 2010

26.7K
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex
09:23

Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

Published on: June 30, 2019

12.6K

Area of Science:

  • * Zoology and molecular biology research.
  • * Development of novel preservation techniques for biological samples.

Background:

  • * Traditional fixatives like formalin, ethanol, and RNAlater have limitations for preserving both morphology and molecular integrity, especially for non-model organisms studied in the field.
  • * Field research often involves limited specimen availability, necessitating single-fixative solutions for comprehensive analysis.
  • * Existing methods struggle to balance morphological preservation with high-quality nucleic acid extraction for advanced molecular techniques.

Purpose of the Study:

  • * To evaluate the efficacy of KINFix, a new alcohol-based fixative, for simultaneous preservation of morphology and molecules.
  • * To assess KINFix's suitability for electron microscopy, RNA preservation, and single-cell RNA sequencing (scRNA-seq).
  • * To determine KINFix's utility for field-based studies on non-model invertebrates.

Main Methods:

  • * Testing KINFix on four different invertebrate species from various spiralian phyla.
  • * Evaluating KINFix's performance in electron microscopy for morphological preservation.
  • * Assessing RNA integrity after 3 months of KINFix storage.
  • * Analyzing cell dissociation suitability for scRNA-seq applications.

Main Results:

  • * KINFix effectively preserves morphology and cellular integrity, suitable for electron microscopy and cell dissociation.
  • * RNA integrity is maintained for over 3 months in KINFix, comparable to standard fixatives.
  • * The fixative supports both morphological and molecular analyses from a single sample.
  • * KINFix demonstrates suitability for scRNA-seq applications in invertebrates.

Conclusions:

  • * KINFix is a versatile, cost-effective fixative enabling simultaneous morphological and molecular analyses.
  • * It is particularly valuable for field research on non-model organisms with limited sample availability.
  • * KINFix supports advanced molecular techniques like scRNA-seq, expanding research possibilities.
  • * Optimization may be needed for specific species and tissues, but KINFix offers a significant advancement in sample preservation.