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Related Experiment Video

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Generation of Multicellular Human Primary Endometrial Organoids
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Simple and Rapid Model to Generate Differentiated Endometrial Floating Organoids.

Adriana Bajetto1, Alessandra Pattarozzi1, Alessandro Corsaro1

  • 1Section of Pharmacology, Department of Internal Medicine, University of Genova, Genova, Italy.

Bio-Protocol
|February 12, 2026
PubMed
Summary

Researchers developed a novel 3D endometrial organoid model that includes stromal cells, preserving crucial epithelial-stromal interactions for studying endometrial physiology and disease.

Keywords:
Endometrial organoidsEndometrium differentiationEpithelial cellsIn vitro 3D cultureStromal cells

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Area of Science:

  • Biomedical Engineering
  • Reproductive Biology
  • Stem Cell Biology

Background:

  • Three-dimensional (3D) organoids are valuable tools for modeling tissue physiology and pathology.
  • Current endometrial organoid models lack stromal cells, limiting the study of essential epithelial-stromal interactions.
  • Epithelial-stromal interactions are critical for normal endometrial function and disease pathogenesis.

Purpose of the Study:

  • To develop a novel 3D endometrial organoid model that incorporates stromal cells.
  • To enable the study of epithelial-stromal interactions in the endometrium in vitro.
  • To create a more physiologically relevant model for endometrial research.

Main Methods:

  • Endometrial organoids were generated in floating Matrigel droplets using a standard culture medium.
  • The model was designed to facilitate the co-culture of epithelial and stromal cells.
  • Characterization involved assessing gene expression and structural integrity.

Main Results:

  • The novel floating organoids formed gland-like structures with a lumen and apicobasal polarity.
  • These organoids retained both epithelial and stromal cell populations in close proximity.
  • The model produced differentiated organoids without requiring external hormonal stimulation, mimicking in vivo conditions.

Conclusions:

  • A simple and rapid method for generating 3D endometrial organoids with preserved epithelial-stromal interactions was established.
  • This model allows for the investigation of reciprocal communication between endometrial epithelial and stromal cells.
  • The developed organoids represent a significant advancement for studying endometrial biology and disease.