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Published on: June 29, 2017
Potassium ion fluorescent sensing in chemically stimulated mammalian cells using a GINKO2-encapsulated sensor
Azumi Sako1, Yusuke Nasu2,3,4, Akiko Obata1
1Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi 466-8555, Japan. toshitcm@nitech.ac.jp.
Abstract:
The nanofibre interior of electrospun fibremats provides an effective platform for immobilising sensor proteins for biomolecule detection. We developed fibremats with GINKO2-a fluorescent K+ sensor-encapsulated in nanofibres. This method enabled reversible K+ detection even with protease and real-time monitoring of changes in the K+ concentration secreted by chemically stimulated mammalian cells.
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