MALDI-FISH for Colocalization of Brominated Metabolites and Pseudovibrio spp. in Aplysina Tissue
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Updated: Feb 13, 2026

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms
Published on: January 11, 2015
Allyson McAtamney1, Cayla Lagousis2, Bradley M Colquitt2
1Department of Chemistry and Biochemistry, University of California, Santa Cruz, 1156 High St., Santa Cruz, California 95064, United States.
Abstract:
Bromotyrosine-containing natural products have been isolated from marine sponges. While studying the uptake of engineered bacterial strains by sponges, we observed the production of fistularin-3 in the sponge. To explore these results, we employed multimodal imaging to colocalize metabolites with fluorescent in situ hybridization (FISH) probes. Using mass spectrometry imaging (MSI), we measured fistularin-3 and mapped this against the FISH probes to locate the bacteria. A recent study reported that P. brasiliensis produced fistularin-3 in pure culture, but these data have not yet been reproduced. We employed MSI and FISH to colocalize the fistularin-3 spatial distribution with P. brasiliensis in sponge tissue.
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