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Related Experiment Video

Updated: Feb 13, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
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Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

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Germacrone Alleviates Cisplatin-Induced Nephrotoxicity by Activating PI3K/Akt Signaling, Inhibiting Ras/MAPK

Liujie Zheng1, Yinhua Ni1, Suyao Xie1

  • 1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|February 12, 2026
PubMed
Summary

Germacrone, a compound from Rhizoma Curcuma, effectively prevents cisplatin-induced acute kidney injury (AKI) by reducing inflammation and apoptosis. It protects kidneys without compromising chemotherapy efficacy, offering potential as an adjuvant therapy.

Keywords:
acute kidney injuryapoptosisgermacroneinflammationmacrophage polarizationrenoprotection

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Area of Science:

  • Nephrology
  • Pharmacology
  • Cancer Therapy

Background:

  • Cisplatin chemotherapy can cause severe acute kidney injury (AKI), necessitating protective strategies.
  • Germacrone, a sesquiterpenoid from Rhizoma Curcuma, exhibits anti-inflammatory, antiapoptotic, and antioxidant properties.

Purpose of the Study:

  • To investigate the renoprotective effects of germacrone against cisplatin-induced AKI.
  • To elucidate the underlying molecular mechanisms of germacrone's protective action.
  • To assess if germacrone affects cisplatin's anticancer efficacy.

Main Methods:

  • Cisplatin-induced AKI and AKI-CKD transition mouse models were utilized.
  • In vitro studies were conducted using HK-2 and Raw264.7 cells.
  • RNA sequencing identified key signaling pathways involved.

Main Results:

  • Germacrone significantly reduced serum creatinine and BUN levels, improving renal pathology in cisplatin-treated mice.
  • Germacrone attenuated cisplatin-induced apoptosis and inflammation in vivo and in vitro.
  • Germacrone modulated the PI3K/AKT and Ras/MAPK pathways and promoted M2 macrophage polarization.

Conclusions:

  • Germacrone demonstrates significant renoprotective effects against cisplatin-induced AKI through anti-inflammatory and antiapoptotic mechanisms.
  • The protective effects involve the PI3K/AKT and Ras/MAPK pathways and influence macrophage polarization.
  • Germacrone is a promising adjuvant therapy for mitigating cisplatin nephrotoxicity without hindering anticancer activity.