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Updated: Feb 13, 2026

Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures
Published on: May 9, 2008
Spectrin coordinates cell shape and signaling essential for epidermal differentiation
Arad Soffer1, Aishwarya Bhosale2,3, Roohallah Ghodrat2,3
1Gray School of Medical Sciences, Gray Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, Israel.
None:
Cell shape and fate are tightly linked, yet how the cortical cytoskeleton integrates regulation of shape and fate remains unclear. Using the multilayered epidermis as a paradigm for cell shape-guided changes in differentiation, we identify spectrin as an essential organizer of the actomyosin cortex to integrate transitions in cell shape with spatial organization of signaling. Loss of αII-spectrin (Sptan1) in mouse epidermis altered cell shape in all layers and impaired differentiation and barrier formation. High-resolution imaging and laser ablation revealed that E-cadherin organizes gradients of cortical actin and spectrin into layer-specific submembranous networks with discrete structural and mechanical properties that coordinate cell shape and fate. This layer-specific organization dissipates tension and, in upper layers, retains activated growth factor receptor EGFR and the calcium channel TRPV3 at the membrane to induce terminal differentiation. Together, these findings reveal how polarized organization of the cortical cytoskeleton directs transitions in cell shape and cell fate at the tissue scale necessary to establish epithelial barriers.
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