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Updated: Feb 13, 2026

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Effects of N-Acetylcysteine and Alpha-Ketoglutarate on OVCAR3 Ovarian Cancer Cells: Insights from Integrative

Yasaman Khamineh1, Sanaz Panahi-Alanagh1, Samaneh Zolghadri2

  • 1Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran 1983969411, Iran.

Cells
|February 12, 2026
PubMed

Insights

N-acetylcysteine (NAC) and alpha-ketoglutarate (AKG) combination therapy shows promise for ovarian cancer treatment. This study found that combining NAC and AKG significantly inhibits ovarian cancer cell growth, migration, and colony formation in vitro.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Ovarian cancer is a leading cause of cancer mortality in women, necessitating innovative combination therapies.
  • N-acetylcysteine (NAC) and alpha-ketoglutarate (AKG) possess anticancer properties, but their combined efficacy in ovarian cancer is not well understood.

Purpose of the Study:

  • To investigate the synergistic effects of NAC and AKG on ovarian cancer cells using an integrative network pharmacology and in vitro approach.
  • To identify common molecular targets and pathways affected by NAC and AKG in ovarian cancer.

Main Methods:

  • Network pharmacology analysis to identify shared targets between NAC, AKG, and ovarian cancer genes.
  • Protein-protein interaction network construction and pathway enrichment analysis (GO, KEGG).
  • In vitro validation of combined NAC and AKG effects on OVCAR3 cell viability, apoptosis, migration, and clonogenic capacity.

Main Results:

  • Network analysis revealed 70 shared target genes involved in apoptosis, cellular stress, and cell migration pathways.
  • Combined NAC (10 mM) and AKG (100 µM) treatment significantly reduced OVCAR3 cell viability.
  • Combination therapy markedly suppressed ovarian cancer cell migration and colony formation compared to single agents.

Conclusions:

  • The combination of NAC and AKG demonstrates enhanced inhibitory effects on ovarian cancer cell growth and motility.
  • This synergistic effect suggests potential for a novel therapeutic strategy against ovarian cancer.
  • Further research into the molecular mechanisms underlying NAC and AKG synergy is warranted.
Keywords:
N-acetylcysteinealpha-ketoglutarateapoptosiscell migrationcell viabilitynetwork pharmacologyovarian cancer

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