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Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Osmoregulation in Fishes02:32

Osmoregulation in Fishes

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When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
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The Role of Actin and Myosin in Non-muscle Cells01:10

The Role of Actin and Myosin in Non-muscle Cells

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Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
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Chemistry of the Cell02:58

Chemistry of the Cell

48.4K
The cell is chemically composed of water, organic molecules and inorganic ions.
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
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Batteries and Fuel Cells03:12

Batteries and Fuel Cells

31.1K
A battery is a galvanic cell that is used as a source of electrical power for specific applications. Modern batteries exist in a multitude of forms to accommodate various applications, from tiny button batteries such as those that power wristwatches to the very large batteries used to supply backup energy to municipal power grids. Some batteries are designed for single-use applications and cannot be recharged (primary cells), while others are based on conveniently reversible cell reactions that...
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Updated: Feb 13, 2026

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
07:36

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Published on: August 28, 2018

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In Vitro Fish Cell Culture: From Primary Muscle Cells to Cell-Based Meat in Cyprinidae.

Piyathip Setthawong1, Chanati Jantrachotechatchawan2, Suppakorn Netmanee3

  • 1Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Biology
|February 12, 2026
PubMed
Summary
This summary is machine-generated.

Cultivating fish muscle cells offers a sustainable alternative to wild fish. This review details methods for fish cell culture, supporting alternative protein development and aquatic research.

Keywords:
Cyprinidaecell culturecultured meatfish muscle cell

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Area of Science:

  • Aquaculture and Cell Biology
  • Sustainable Food Production

Background:

  • Wild fish stocks are declining due to increased demand and climate change.
  • Cultivated fish meat presents a sustainable food alternative.
  • Fish muscle provides high-quality protein with balanced nutrients and low fat.

Purpose of the Study:

  • To review fish muscle structure and physiology.
  • To highlight methods for establishing primary fish muscle cell cultures.
  • To discuss advancements in fish cell culture for sustainable bioproduction.

Main Methods:

  • Explant outgrowth and enzymatic dissociation for cell culture initiation.
  • Optimization of environmental conditions and growth media.
  • Characterization of Cyprinidae muscle cell lines using morphology, gene expression, and karyotyping.

Main Results:

  • Detailed methods for establishing and characterizing fish muscle cell lines are presented.
  • Recent advances in 3D culture systems and bioreactors for large-scale production are discussed.
  • Challenges such as lack of standardized cell lines and serum dependence are identified.

Conclusions:

  • Fish muscle cell culture is crucial for developing alternative protein sources.
  • It also supports aquatic health research, disease modeling, and sustainable bioproduction.
  • Future progress requires innovations in serum-free media and cost-effective protocols.