Exploring the potential of indigenous coal mine isolates Pseudomonas aeruginosa (PM55) and Enterobacter cloacae (PM56) for Cr(VI) bioremediation and plant growth promotion
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Summary
This summary is machine-generated.Two bacterial strains, Pseudomonas aeruginosa (PM55) and Enterobacter cloacae (PM56), were isolated and found to tolerate high levels of hexavalent chromium (Cr(VI)). These strains show potential for bioremediation of Cr(VI) and promoting plant growth in contaminated soils.
Area Of Science
- Environmental Microbiology
- Bioremediation
- Plant-Microbe Interactions
Background
- Hexavalent chromium (Cr(VI)) is a toxic heavy metal pollutant originating from industrial activities, particularly coal mining.
- Effective bioremediation strategies are crucial for mitigating Cr(VI) contamination in soil and water ecosystems.
- Developing microbial solutions for Cr(VI) remediation is essential for environmental sustainability and agricultural productivity.
Purpose Of The Study
- To isolate and characterize Cr(VI)-tolerant bacterial strains from coal mine soil for bioremediation applications.
- To evaluate the plant growth-promoting abilities of selected strains under Cr(VI) stress.
- To investigate the biosorption mechanisms and capacity of these bacteria for Cr(VI) removal.
Main Methods
- Isolation and screening of bacterial strains from Chakwal coal mine soil for Cr(VI) tolerance.
- Identification of plant growth-promoting traits (EPS, IAA, ACC-deaminase) and the ChrR reductase gene.
- Batch experiments to determine optimal conditions (pH, temperature, time, Cr(VI) concentration) for biosorption.
- Analysis of biosorption using Langmuir, Freundlich, Pseudo-second-order, and intra-diffusion models.
- Characterization of biosorption using FTIR, SEM, and EDX techniques.
Main Results
- Two bacterial strains, Pseudomonas aeruginosa (PM55) and Enterobacter cloacae (PM56), were isolated, tolerating up to 350 ppm Cr(VI).
- Both strains exhibited plant growth-promoting activities (EPS, IAA, ACC-deaminase) under Cr(VI) stress and possessed the ChrR reductase gene.
- Pseudomonas aeruginosa (PM55) demonstrated a higher Cr(VI) biosorption capacity than Enterobacter cloacae (PM56), with adsorption fitting Langmuir and Freundlich models.
- FTIR, SEM, and EDX analyses confirmed the biosorption of Cr(VI) by the selected bacterial strains.
- Pseudomonas aeruginosa (PM55) effectively promoted the growth of linum seedlings under Cr(VI) stress.
Conclusions
- Pseudomonas aeruginosa (PM55) and Enterobacter cloacae (PM56) are promising candidates for the bioremediation of Cr(VI)-contaminated environments.
- The identified strains possess multiple mechanisms for Cr(VI) tolerance and plant growth promotion, contributing to sustainable agriculture.
- Pseudomonas aeruginosa (PM55) shows significant potential for Cr(VI) remediation and enhancing crop resilience in polluted soils.
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