Response surface methodology based Box-Behnken Design for statistical optimization of Curcuma caesia mediated copper
Sakshi Sharma1, Saurabh Srivastava2, Abhishek Chaudhary1
1Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh, 173234, India.
Biochemical and Biophysical Research Communications
|February 12, 2026
Summary
Green synthesis of copper oxide nanoparticles using Curcuma caesia extract offers a novel approach to overcome bioavailability issues. These nanoparticles show significant antioxidant, antibacterial, anti-inflammatory, and anti-diabetic properties for pharmaceutical applications.
Area of Science:
- Nanotechnology
- Green Chemistry
- Pharmacology
Background:
- Curcuma caesia possesses therapeutic potential but faces limitations in pharmaceutical use due to poor solubility, stability, and bioavailability.
- Developing novel drug delivery systems is crucial to harness the full therapeutic benefits of natural compounds.
- Nanoparticles offer enhanced properties for drug delivery and therapeutic applications.
Purpose of the Study:
- To synthesize copper oxide nanoparticles using an eco-friendly green synthesis method with Curcuma caesia extract.
- To optimize the synthesis process and characterize the resulting nanoparticles.
- To evaluate the therapeutic potential of Curcuma caesia-mediated copper oxide nanoparticles through various biological assays.
Main Methods:
- Green synthesis of copper oxide nanoparticles using Curcuma caesia extract as a reducing and stabilizing agent.
- Optimization using one variable at a time and Response Surface Methodology (Box-Behnken Design).
- Characterization via UV-Vis spectroscopy, FTIR, SEM, TEM, and XRD; biological assays including DPPH, antibacterial, anti-inflammatory, and anti-diabetic tests.
Main Results:
- Synthesized spherical copper oxide nanoparticles (50-70 nm) with Surface Plasmon Resonance at 430 nm.
- FTIR confirmed the role of Curcuma caesia bioactives in nanoparticle synthesis and capping.
- Demonstrated significant antioxidant (IC50: 13.037 µM), antibacterial, anti-inflammatory (62-72%), and anti-diabetic (36.62-59.79%) activities.
Conclusions:
- Curcuma caesia mediated copper oxide nanoparticles are effectively synthesized via a green approach.
- The synthesized nanoparticles exhibit promising multi-target therapeutic activities.
- These findings support the potential application of these nanoparticles in the biomedical and pharmaceutical fields.
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