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Updated: Feb 14, 2026
Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
Published on: September 6, 2018
Generation and characterization of iPSC lines (SPPHIi005-A, SPPHIi006-A, SPPHIi007-A) from a CRB1-mutation associated
Ning Xiao1, Weijia Wu1, Lingxue Gong2
1Genetic diseases Key Laboratory of Sichuan Province and the Center for Medical Genetics, Department of Laboratory Medicine, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, School of medicine, University of Electronic Science and Technology of China, Chengdu 610054, P.R. China; Human Disease Genes Key Laboratory of Sichuan Province and Institute of Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
Abstract:
Mutations in the Crumbs homolog 1 (CRB1) gene are associated with autosomal recessive retinal pigmentosa (RP). We derived two human-induced pluripotent stem cell (hiPSC) lines, SPPHIi006-A and SPPHIi007-A, from a consanguineous family affected by RP. These lines carry either a homozygous or heterozygous CRB1 variant c.1997 T > A (p.V666D). Additionally, we established a hiPSC line, SPPHIi005-A, from a healthy family member without the CRB1 mutation to serve as a normal control (HC). All three hiPSC lines demonstrated normal karyotypes, cell morphology, hiPSC markers, and differentiation into three germ layers. These hiPSC lines offer valuable models for exploring therapeutic strategies for CRB1-associated RP.
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