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Neuroprotective effects of pepsin-hydrolyzed ovotransferrin on H2O2-induced oxidative damage in SH-SY5Y neuronal
Hyeong-Jin Kim1, Seung-Woo Yu1, Kee-Tae Kim2
1Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea.
Abstract:
Neurodegenerative diseases are considered critical disorders due to the absence of effective treatments and serious damage to brain function. Ovotransferrin, an egg white protein, and its hydrolysate obtained using proteolytic enzymes have been reported to have beneficial effects on various bioactive functions. Therefore, the purpose of this study was to investigate the neuroprotective effects of enzymatically hydrolyzed ovotransferrin in SH-SY5Y cells exposed to oxidative stress. Hydrolyzed ovotransferrin showed higher antioxidative activities than pure ovotransferrin. Furthermore, 0.5 mg/mL of pepsin-derived ovotransferrin hydrolysate (OHPE) exhibited the highest cell viability (81.89%), and OHPE effectively reduced cellular ROS accumulation (72%) and decreased total apoptosis rate (46.41%). These anti-apoptotic and antioxidative effects were influenced by the regulation of the mRNA and protein expression of Bax, Bcl-2, caspase-9, caspase-3, Nrf2, and HO-1. Additionally, nine peptides comprising OHPE were identified by LC-MS/MS and compared with the sequences reported in DFBP to explore their potential as antioxidants. These results indicate that OHPE can be used as a nature-derived compound to prevent neurodegenerative diseases by inhibiting ROS interactions and regulating protein expression in neuronal cells.
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