Related Experiment Video
Updated: Feb 14, 2026

Western Blotting: Sample Preparation to Detection
Published on: October 14, 2010
Quantitative Western blot assay for the detection of chicken serum albumin (Gal d 5)
Yaqi Zhao1, Aravind Kumar Bingi1, Qinchun Rao1
1Center for Integrative Nutrition and Food Research, Department of Health, Nutrition, and Food Sciences, Florida State University, 120 Convocation Way, Tallahassee, FL 32306, USA.
Abstract:
Egg is one of the Big-Nine allergenic foods in the U.S., and accurate detection of its allergenic proteins is critical for food safety. Chicken serum albumin (CSA, Gal d 5), an allergen found in egg yolk, chicken meat, and avian-derived materials, remains under-detected in food products. This study aimed to characterize CSA and develop a quantitative Western blot (qWB) assay for the detection of CSA. After characterization, (1) high temperature (>69.61 °C) decreased CSA extractability, (2) in vitro allergenicity was detected by human plasma, and (3) CSA was verified in egg white. A monoclonal antibody 3H4E9 was characterized as specific to poultry serum albumin. The optimized qWB assay exhibited good selectivity (specific to poultry species), working range (0.3-20 ppm), and sensitivity (ppm level). This assay enables sensitive and reliable CSA detection on food contact surfaces, facilitating risk assessment of undeclared egg yolk residues and improving food safety management practices.
More Related Videos
Related Concept Videos
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
5-Number Summary
In a box plot, the minimum and maximum data values represent the lower and upper whiskers in the graph, and the median is designated as the center of the box in the chart. The first quartile and third...
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Serum Studies: Renal Function Tests
Serum Laboratory Studies, Stool Test, Breath Test
Quantitative Analysis
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...

