Related Experiment Video
Updated: Feb 14, 2026

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
Published on: March 10, 2017
A brief guide to isolating primary hepatocytes from mice
Jisoo Song1, Garam An2, Gwonhwa Song3
1Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, Republic of Korea; Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Abstract:
The isolation of primary hepatocytes, which are liver parenchymal cells, is a valuable tool in biomedical research for exploring liver physiology and disease mechanisms. However, obtaining hepatocytes with high viability can be challenging owing to the technical complexity of the procedure and fragility of the cells. Here, we present an optimized protocol, along with key considerations, for isolating hepatocytes from mice with high yield and viability, thereby contributing to liver-related biomedical research.
Related Concept Videos
Primary Active Transport
Primary Active Transport
Primary and Secondary Growth in Roots and Shoots
Primary Production
Primary Distribution
Nomenclature of Primary Amines

