Related Experiment Video
Updated: Feb 14, 2026

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
Published on: October 25, 2017
An insight into the characterization of multidrug resistant Salmonella Minnesota isolated from poultry in Brazil
Dany Mesa1, Stella Almeida1, Damaris Krul1
1Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Paraná, Brazil.
Abstract:
Salmonella enterica subspecies enterica serovar Minnesota (S. Minnesota) is an emerging serovar in Brazilian poultry production. This study aimed to characterize the antimicrobial resistance profile of 20 S. Minnesota strains isolated from broiler chicken litter in Paraná, southern Brazil, between 2021 and 2023, and to confirm these findings with whole-genome sequencing. Antimicrobial susceptibility testing showed all isolates were multidrug resistant. High resistance rates were observed against ciprofloxacin (100%), ceftazidime (95%), and gentamicin (70%). Genomic analysis confirmed the resistance profiles, revealing key resistance determinants: the beta-lactamase gene blaCMY-2 was present in all isolates; parC p.T57S mutation was present in all isolates, along with plasmid-borne qnrB genes in some, correlating with ciprofloxacin resistance; and aminoglycoside resistance genes such as aac(6')-Iy, aph(3)-Ia, and aadA1 were detected. Additionally, all isolates belonged to sequence type ST548 and carried tet(A), sul2, and acrB resistance genes. A major finding was a discrepancy in in-silico serotyping. While SISTR identified all strains as S. Minnesota, SeqSero2 identified one as S. Minnesota and 19 as inconclusive, a difference that highlights the limitations of serological and in silico methods. These results emphasize the spread of MDR S. Minnesota in poultry and the necessity of genomic surveillance for accurate resistance monitoring and public health protection.
More Related Videos
09:29In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging
Published on: April 28, 2017
23:06The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
Published on: August 11, 2008
Related Concept Videos
Resistivity
Resistance
Equivalent Resistance
Resistance and Conductance
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
Rolling Resistance
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
Vascular Resistance
The primary determinants of vascular resistance are vessel diameter, blood viscosity, and vessel length. Among these, vessel diameter plays the most significant role due to the fourth power relationship described by...