Light sheet microscopy imaging dataset of CAR-T-cell-mediated cytotoxicity

Research on CAR-T cell states is crucial for understanding the mechanisms of immunotherapy. Previous studies in live cells have been primarily limited by phototoxicity, resolution, and throughput, making it difficult to conduct further research and observations on cell states. To enable more detailed studies of cell states, we developed a microscopy imaging system with subcellular resolution, low phototoxicity, high imaging throughput, and automated data reconstruction. Using this system, we have generated and shared over 400 image sets that capture the cytotoxic effects of CAR-T cells on tumor cells. The data provide an isotropic spatial resolution of 320 nm, a temporal resolution of up to 2.5 seconds per volume, and long-term observations spanning up to 5 hours. This study reports an imaging system that fills an essential gap in the field, offers valuable insights into the cytotoxic processes of CAR-T cells, and significantly advances research in this area.