Related Experiment Video
Updated: Feb 14, 2026
08:56
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
7.9K
QTL mapping, breeding, and debugging Saccharomyces cerevisiae strains through Reiterated Mass Selection and
Lucas Souza de Bem1,2, Joneclei Alves Barreto1,3, Diego Trindade de Souza4
1Institute for Research in Bioenergy, São Paulo State University, Rio Claro, 13500-230, Brazil.
Biotechnology for Biofuels and Bioproducts
|February 12, 2026
Summary
We developed Reiterated Mass Selection and backcrosSing (ReMaSSing) to improve yeast tolerance to lignocellulosic hydrolysates (LCHs). This method efficiently identifies adaptive alleles, enhancing yeast fermentation capabilities for biofuel production.
Area of Science:
- Biotechnology
- Yeast genetics
- Metabolic engineering
Background:
- Second-generation ethanol production from lignocellulosic hydrolysates (LCHs) is hindered by yeast fermentation inhibitors.
- Quantitative trait loci (QTL) mapping is crucial for identifying yeast alleles that enhance LCH tolerance.
- Current QTL mapping methods are labor-intensive and require extensive screening and breeding.
Purpose of the Study:
- To develop an efficient method for QTL mapping to identify adaptive alleles for LCH tolerance in Saccharomyces cerevisiae.
- To enhance the fermentation capabilities of yeast strains for industrial biofuel production.
Main Methods:
- Developed Reiterated Mass Selection and backcrosSing (ReMaSSing) for QTL mapping and strain improvement.
- Applied ReMaSSing to yeast populations derived from crossing stress-resistant and laboratory strains.
- Utilized whole-genome sequencing and bulked segregant analysis for QTL identification.
- Performed allele swapping and competition assays to validate identified QTL.
Main Results:
- ReMaSSing successfully identified key QTL linked to LCH tolerance, including alleles influencing growth and mitochondrial function.
- Selected yeast strains exhibited over 20% improvement in LCH tolerance and growth compared to parental strains.
- ReMaSSing facilitated the "debugging" of the laboratory strain background by purging detrimental variants.
- Developed improved yeast strains with enhanced LCH tolerance and xylose fermentation capabilities.
Conclusions:
- ReMaSSing provides a practical and efficient protocol for QTL mapping and the generation of improved yeast strains.
- Recombinant yeast populations derived from ReMaSSing demonstrate superior LCH tolerance and growth.
- ReMaSSing is a powerful tool for engineering industrial yeast strains with integrated desirable traits for biofuel production.
Related Concept Videos
01:59Plant Breeding and Biotechnology
21.9K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
21.9K
01:32What is Natural Selection?
129.8K
Natural selection is an evolutionary process in which individuals with survival-promoting traits reproduce at higher rates. These favorable traits become more common within a population or species. Naturally selected traits initially arise via random genetic mutations. In order for selection to occur, there must be variation within a population, the trait controlling the variation must be heritable, and there must be an evolutionary advantage for variation in the trait.
129.8K
01:52Atomic Mass
70.6K
Atoms — and the protons, neutrons, and electrons that compose them — are extremely small. For example, a carbon atom weighs less than 2 × 10−23 g. When describing the properties of tiny objects such as atoms, we use appropriately small units of measure, such as the atomic mass unit (amu). The amu was originally defined based on hydrogen, the lightest element, then later in terms of oxygen. Since 1961, it has been defined with regard to the most abundant isotope of carbon, atoms of which...
70.6K
01:54Molar Mass
87.1K
The identity of a substance is defined not only by the types of atoms or ions it contains but by the quantity of each type of atom or ion. For example, water, H2O, and hydrogen peroxide, H2O2, are alike in that their respective molecules are composed of hydrogen and oxygen atoms. However, because a hydrogen peroxide molecule contains two oxygen atoms, as opposed to the water molecule, which has only one, the two substances exhibit very different properties.
87.1K
01:19Thermal Strain
2.9K
Thermal strain is a concept that arises when we consider how temperature changes affect structures. Unlike the conventional assumption that structures remain constant under load, real-world scenarios often involve temperature fluctuations that can significantly impact these structures. Consider a homogeneous rod with a uniform cross-section resting freely on a flat horizontal surface. If the rod's temperature increases, the rod elongates. This elongation is proportional to the temperature...
2.9K
01:20Shearing Strain
1.5K
The shearing strain represents a cubic element's angular change when subjected to shearing stress. This type of stress can transform a cube into an oblique parallelepiped without influencing normal strains. The cubic element experiences a significant transformation when exposed solely to shearing stress. Its shape alters from a perfect cube into a rhomboid, clearly demonstrating the effect of shearing strain. The degree of this strain is considered positive if it reduces the angle between the...
1.5K