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Updated: Feb 14, 2026

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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
Published on: October 15, 2019
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Porcine Skeletal Muscle-Specific lncRNA-ssc.37456 Regulates Myoblast Proliferation and Differentiation
Xia He1,2, Yangshuo Hu1, Yangli Pei1
1School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Animals : an Open Access Journal From MDPI
|February 13, 2026
Summary
Long-chain non-coding RNA (lncRNA)-ssc.37456 promotes skeletal muscle development in pigs by regulating cell proliferation and differentiation. This finding offers new targets for muscle biology and breeding.
Area of Science:
- Molecular Biology
- Genetics
- Animal Science
Background:
- Long-chain non-coding RNAs (lncRNAs) are crucial for skeletal muscle growth.
- Systematic studies on lncRNAs in porcine skeletal muscle development are limited.
Purpose of the Study:
- Identify and functionally characterize lncRNA-ssc.37456 in porcine skeletal muscle development.
- Investigate the molecular mechanisms underlying its role in myogenesis.
Main Methods:
- Utilized porcine skeletal muscle regeneration and primary myoblast differentiation models.
- Performed knockdown and overexpression experiments in vitro.
- Analyzed gene expression (mRNA/protein), cell proliferation (EdU), and myotube formation.
Main Results:
- lncRNA-ssc.37456 expression correlated positively with differentiation markers (MYHC) and negatively with proliferation markers (PAX7).
- Knockdown promoted proliferation and inhibited differentiation; overexpression had opposite effects.
- lncRNA-ssc.37456 is cytoplasmically localized, suggesting a ceRNA mechanism involving miRNAs.
Conclusions:
- lncRNA-ssc.37456 is a key regulator of the proliferation-to-differentiation transition in porcine skeletal muscle cells.
- Provides insights into muscle development and potential targets for molecular breeding.
Keywords:
lncRNA-ssc.37456muscle specificityporcine primary myoblast proliferation and differentiationMore Related Videos
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