Three-Dimensional Reconstruction of the Equine Palmar Metacarpal Region Using E12 Plastinated Sections

Digital technologies have improved the visualization of anatomical structures for veterinary education and clinical practice. In this study, a detailed three-dimensional anatomical model of the equine palmar metacarpal region was generated using E12-based epoxy sheet plastination combined with digital reconstruction in Amira^® V5.6 software. Serial cross-sections of the metacarpal region provided high-resolution visualization of bones, tendons, ligaments, nerves, vessels, fasciae, and synovial structures, with minimal shrinkage or deformation, ensuring improved anatomical accuracy. These sections were digitized, aligned, and manually segmented to accurately delineate anatomical boundaries, particularly in areas of low contrast. The resulting three-dimensional model represents the topographical relationships of key structures, including palmar nerves and vessels, the palmar fascia with the metacarpal flexor retinaculum (MFR), and the common synovial sheath (Vag. synovialis communis mm. flexorum, CSS). The model allows rotation and selective visualization of individual structures, facilitating examination from multiple perspectives. This combined plastination-digital approach provides an accurate anatomical reference with value for veterinary anatomy education, clinical training, surgical planning, and research on equine musculoskeletal disorders.