Synergistic Modulation of Nitrogen and Chemical Regulation: Balancing Photosynthesis and Lodging Resistance for...

While increasing planting density is a viable strategy for enhancing maize yield, it concurrently elevates the risks of lodging and accelerated leaf senescence due to intensified inter-plant competition, which can ultimately compromise yield stability. A field experiment was conducted in Heilongjiang Province and the study investigated two maize cultivars, JNK728 (Jingnongke 728) and SD5 (Saide 5), under high-density planting conditions (90,000 plants ha^-1). The treatments were arranged in a factorial design, incorporating four nitrogen levels (0, 120, 240, and 360 kg N ha^-1) in combination with the presence or absence of a chemical regulator (30% diethyl aminoethyl hexanoate · ethephon), with water serving as the control. Results demonstrated that the integration of 240 kg N ha^-1 with chemical regulation significantly enhanced photosynthetic capacity-elevating chlorophyll content (SPAD), net photosynthetic rate (Pn), and activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) and phosphoenolpyruvate carboxylase (PEPCase)-while improving canopy structure through increased leaf area index (LAI) and optimized light distribution. This strategy also reinforced lodging resistance by optimizing plant morphology (reducing plant height and center of gravity), strengthening basal internodes (increasing stem diameter, dry weight per unit length, and mechanical strength), and promoting accumulation of stem structural components (cellulose, hemicellulose, lignin). Additionally, it facilitated post-anthesis nitrogen translocation to grains and up-regulated key nitrogen metabolism enzymes (glutamate synthetase-GS, glutamate dehydrogenase-GDH, and glutamate-pyruvate transaminase-GPT), thereby boosting nitrogen use efficiency. In contrast, excessive nitrogen (360 kg N ha^-1) suppressed these benefits and increased lodging. Consequently, the combined application of 240 kg N ha^-1 with chemical regulation achieved the highest yield, proving an effective approach for synergistically enhancing photosynthesis, lodging resistance, and nitrogen utilization in high-density maize systems.