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Published on: May 23, 2025
Rapid Determination of Molybdenum (VI) in Water Using Phenylfluorone-Modified Test Strips Combined with Colorimetry
Xingping Li1, Daiwei Zhuang2,3, Xiaoling Liu4
1School of Ecological Engineering, Henan Forestry Vocational College, Luoyang 471002, China.
None:
Excessive molybdenum (VI) (Mo (VI)) in water threatens environmental safety and human health, yet rapid on-site methods for Mo (VI) determination remain limited. Here, we propose a rapid method for Mo (VI) determination using phenylfluorone (PF)-modified test strips with dual readouts: visual colorimetry and image-based analysis in the CIELAB (Lab*) color space, and demonstrate its applicability using urban park water samples. Based on visual colorimetry, a standard color card was established, providing a screening range of 0.08 to 0.8 mg L-1 (A blank (0 mg L-1) was used as the baseline reference). Moreover, by the LAB color space, the linear relationship between the color development results of the PF-modified test strip and the A channel conforms to y = 21.08 + 8.82x (R2 = 0.992), with a detection range of 0-0.8 mg L-1. The total detection time was reduced to 2.5 min. To evaluate accuracy in real matrices, influent, midstream, and effluent samples from Chengdu Living Water Park were analyzed, with UV-vis spectrophotometry used as the reference method. The test-strip results agreed well with UV-vis spectrophotometry, with relative errors below 5%. Overall, this study provides a portable, rapid, and accurate method for the detection of Mo (VI) in water, and has potential application prospects in the field of water environment detection in the future.
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