Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structural Protein Function01:56

Structural Protein Function

30.1K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.1K
Structural Protein Function01:56

Structural Protein Function

3.3K
3.3K
Molecular Structure and Acidity02:34

Molecular Structure and Acidity

21.6K
An acid can be deprotonated to form a conjugate base or an anion. If the produced anion is more stable, then the acid is stronger. On the contrary, if the anion is unstable, then the acid is weaker. Hence, to determine the acidity of the compound, the stability of its conjugate base is studied using various factors.
The size effect explains the change in atomic size on acidity. When comparing the acids formed from elements that belong to the same column in the periodic table, their atomic sizes...
21.6K
Acid Strength and Molecular Structure03:05

Acid Strength and Molecular Structure

33.2K
Binary Acids and Bases
In the absence of any leveling effect, the acid strength of binary compounds of hydrogen with nonmetals (A) increases as the H-A bond strength decreases down a group in the periodic table. For group 17, the order of increasing acidity is HF < HCl < HBr < HI. Likewise, for group 16, the order of increasing acid strength is H2O < H2S < H2Se < H2Te. Across a row in the periodic table, the acid strength of binary hydrogen compounds increases with increasing...
33.2K
Fruit Development, Structure, and Function01:58

Fruit Development, Structure, and Function

25.4K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.4K
Lewis Structures of Molecular Compounds and Polyatomic Ions02:54

Lewis Structures of Molecular Compounds and Polyatomic Ions

46.5K
To draw Lewis structures for complicated molecules and molecular ions, it is helpful to follow a step-by-step procedure as outlined:
46.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrathin Dielectric Nanocoatings Enable Multifunctional High-Emissivity Silicon Surfaces.

ACS applied materials & interfaces·2026
Same author

Exploring the Potential of Calebin-A in Targeting Obesity-Related Genes and Pathways.

Journal of cellular and molecular medicine·2026
Same author

Edible Plant-Derived Exosome-like Nanoparticles as Prebiotic Nanocarriers: Gut Microbiota Modulation and Functional Food Potential.

Pharmaceutics·2026
Same author

Silver Nanoparticles and Neurotoxicity: Mechanistic Insights and Recent Experimental Evidence.

Pharmaceutics·2026
Same author

Nature meets immunity: curcumin's role in modulating neutrophil extracellular traps.

Inflammopharmacology·2026
Same author

Evaluating the Effects of Nanocurcumin Supplementation in Type 2 Diabetes Mellitus: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Endocrinology, diabetes & metabolism·2026

Related Experiment Video

Updated: Feb 14, 2026

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight
08:03

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight

Published on: May 31, 2022

5.7K

Optimizing Lactoferrin Isolation for Functional and Structural Integrity: A Molecular Insight.

Ahmet Alperen Canbolat1, Nur Hasret İstekli1, Kadir Yılmaz2

  • 1Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, 17100 Çanakkale, Türkiye.

Molecules (Basel, Switzerland)
|February 13, 2026
PubMed
Summary

Lactoferrin (Lf) isolation methods are reviewed to maintain its bioactive structure and antimicrobial functions. Optimal conditions are crucial for preserving Lf

Keywords:
affinity chromatographybioavailabilitycolostrumisolationlactoferrin

More Related Videos

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

20.4K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.9K

Related Experiment Videos

Last Updated: Feb 14, 2026

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight
08:03

Advanced Self-Healing Asphalt Reinforced by Graphene Structures: An Atomistic Insight

Published on: May 31, 2022

5.7K
Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

20.4K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.9K

Area of Science:

  • Biochemistry
  • Biotechnology
  • Molecular Biology

Background:

  • Lactoferrin (Lf) is an ~80 kDa glycoprotein found in milk and other bodily fluids.
  • Lf possesses diverse bioactivities including antioxidant, antimicrobial, and antiviral properties.
  • Maintaining Lf's structural integrity and bioactivity during isolation is critical for its applications.

Purpose of the Study:

  • To provide a comprehensive framework linking Lf isolation methods to structural integrity, iron-binding domain preservation, and antimicrobial performance.
  • To systematically evaluate ion-exchange, affinity-based, and membrane-based isolation techniques.
  • To outline analytical characterization and biotechnological applications of Lf.

Main Methods:

  • Systematic evaluation of ion-exchange, affinity-based, and membrane-based isolation approaches.
  • Analysis of isolation methodologies concerning structural integrity and functional domains.
  • Review of analytical characterization techniques and biotechnological applications.

Main Results:

  • Inadequate optimization (pH, temperature, ion balance, protease activity) can lead to Lf denaturation and aggregation.
  • Different isolation methods impact the preservation of Lf's structure and iron-binding capabilities.
  • The review synthesizes analytical and functional perspectives to guide Lf isolation procedure selection.

Conclusions:

  • Optimized isolation protocols are essential for sustaining Lactoferrin's bioactivity and functional domains.
  • The choice of isolation method significantly influences the quality and performance of Lf.
  • Understanding these associations facilitates the selection and optimization of Lf isolation for various biotechnological uses.