Related Experiment Video

Updated: Feb 14, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.0K

A Lytic Mosigvirus Phage (GADS24) from a Poultry-Farm Environment: Genome-Resolved Characterization and In Vitro

Jehan Alrahimi1,2, Ghadah Alsubhi1, Alia Aldahlawi1,2

  • 1Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah 22254, Saudi Arabia.

International Journal of Molecular Sciences
|February 13, 2026
PubMed

Insights

A novel bacteriophage, GADS24, effectively targets multidrug-resistant Escherichia coli. This strictly lytic phage shows promise for biocontrol in poultry, addressing critical public health concerns.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Multidrug-resistant Escherichia coli (E. coli) poses significant threats at the poultry-human interface.
  • Strictly lytic bacteriophages are potential candidates for targeted biocontrol of resistant bacteria.

Purpose of the Study:

  • To isolate and characterize a lytic E. coli bacteriophage from poultry waste.
  • To evaluate its biocontrol potential against clinical E. coli isolates.

Main Methods:

  • Isolation and plaque assays of bacteriophage GADS24 from poultry waste.
  • Host range determination against clinical E. coli isolates.
  • Transmission electron microscopy (TEM) for virion morphology.
  • Whole-genome sequencing, annotation, and comparative genomics for taxonomic placement.

Main Results:

  • Bacteriophage GADS24, a strictly lytic agent, was isolated and characterized.
  • GADS24 demonstrated efficacy against 5 out of 10 clinical E. coli isolates.
  • TEM revealed morphology consistent with the Mosigvirus genus.
  • Genomic analysis confirmed GADS24 as a distinct lineage within Mosigvirus, lacking lysogeny genes.

Conclusions:

  • GADS24 is a genome-resolved, strictly lytic Mosigvirus with potential for E. coli biocontrol.
  • Its efficacy against clinical isolates supports further development for poultry-associated infection control.
  • Further studies on phage-host interactions are warranted.
Keywords:
Escherichia coliMosigvirusTevenvirinaebacteriophagebiocontrolcomparative genomicsgenome sequencinghost rangelytic phagepoultry farm

More Related Videos

Following Cell-fate in E. coli After Infection by Phage Lambda06:10

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

24.2K
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo08:32

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Published on: October 23, 2016

11.1K

Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
78.2K
Background and Environment Affect Phenotype02:27

Background and Environment Affect Phenotype

Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.7K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
17.1K
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.3K
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
1.8K