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Mismatch Repair01:36

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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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Published on: June 26, 2020

A Metabolites' Interplay Can Modulate DNA Repair by Homologous Recombination.

Valentina Rossi1, Mirco Masi2, Marzia Govoni3

  • 1Department of Medical and Surgical Sciences (DIMEC), Section of General Pathology, University of Bologna, 40126 Bologna, Italy.

International Journal of Molecular Sciences
|February 13, 2026
PubMed
Summary
This summary is machine-generated.

Cellular metabolites lactate and butyrate impact gene expression and cancer drug efficacy. Researchers found these molecules oppositely affect DNA repair, influencing cancer treatment outcomes.

Keywords:
DNA repairbutyratecancer cellshomologous recombinationlactate

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Area of Science:

  • Metabolomics
  • Cancer Biology
  • Molecular Oncology

Background:

  • Small molecules from metabolism and gut bacteria influence gene expression and cellular functions.
  • Lactate is linked to cancer proliferation and drug resistance, while butyrate exhibits anticancer properties.

Purpose of the Study:

  • To investigate the interplay between lactate and butyrate in modulating anticancer drug efficacy.
  • To explore how these metabolites affect gene expression in cancer cells.

Main Methods:

  • Utilized pancreatic and triple-negative mammary adenocarcinoma cell lines.
  • Analyzed the impact of lactate and butyrate on gene expression related to DNA repair.
  • Assessed the modulation of homologous recombination and response to PARP inhibitors.

Main Results:

  • Lactate and butyrate oppositely modulated key genes in DNA repair by homologous recombination in both cell lines.
  • The efficacy of DNA repair pathways and response to PARP inhibitors were altered.
  • Observed a counteracting effect between lactate and butyrate.

Conclusions:

  • The opposing effects of lactate and butyrate on DNA repair pathways can influence cancer drug response.
  • This metabolite interplay presents a potential strategy to overcome challenges in anticancer therapies.
  • Findings suggest novel therapeutic avenues by manipulating cellular metabolites.