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Updated: Feb 14, 2026

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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A new sensitive derivatization assay of PrC-210 in plasma and tissues using liquid chromatography coupled with tandem

Bryan L Fahl1, Cameron O Scarlett2, William E Fahl1,3

  • 1Obvia Pharmaceuticals Ltd., Madison, WI, USA.

Bioanalysis
|February 13, 2026
PubMed
Abstract

Insights

PrC-210, a reactive oxygen species (ROS) scavenger, shows promise for preventing radiation toxicity, neurodegenerative diseases, and organ injury. A validated LC-MS/MS method quantifies PrC-210 in mouse plasma and tissues.

Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Biochemistry

Background:

  • PrC-210 is a direct-acting reactive oxygen species (ROS) scavenger with potential therapeutic applications.
  • Its efficacy in preclinical models suggests it can prevent ROS-induced conditions like radiation toxicity, neurodegenerative diseases, and ischemia-reperfusion injuries.

Purpose of the Study:

  • To develop and validate a sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying PrC-210.
  • To measure PrC-210 concentrations in mouse plasma and tissue homogenates following various administration routes.

Main Methods:

  • PrC-210's free sulfhydryl group was derivatized with N-ethylmaleimide (NEM) to form a stable PrC-210-NEM conjugate.
  • Acyclovir was used as an internal standard for extraction from plasma and tissue homogenates using acetonitrile/0.1% formic acid.
  • Quantification was performed using a triple quadrupole spectrometer in positive ion mode, validated from 7.5 nM to 5000 nM.

Main Results:

  • The LC-MS/MS method demonstrated high sensitivity, specificity, and accuracy (inter- and intra-assay precision <10%).
  • The limit of quantification for PrC-210-NEM was 2.5 nM.
  • The method successfully measured PrC-210 concentrations in over 126 mice after oral, intraperitoneal, or subcutaneous administration.

Conclusions:

  • A robust LC-MS/MS assay was established for quantifying PrC-210 in biological matrices.
  • This method enables pharmacokinetic and distribution studies of PrC-210 in vivo.
  • The validated assay supports further investigation of PrC-210's therapeutic potential in ROS-related diseases.
Keywords:
N-ethylmaleimideROS (reactive oxygen species)ROS-scavengeraminothiolneurodegenerative

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