Related Experiment Video
Updated: Feb 14, 2026
07:25
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
5.0K
PSLite Online: A Portable, Modern Web Application to Analyze Top-Down Mass Spectrometry Fragmentation Data
Ryan T Fellers1,2, Joseph B Greer1,2, Bryan P Early1,2
1Proteinaceous Inc., Evanston, Illinois, USA.
Journal of Mass Spectrometry : JMS
|February 13, 2026
Summary
PSLite Online is a new web application for analyzing top-down mass spectrometry data in targeted proteoform studies. It offers enhanced usability and collaboration features for proteomics researchers.
Area of Science:
- Proteomics
- Mass Spectrometry
- Computational Biology
Background:
- Top-down mass spectrometry is crucial for proteoform analysis.
- Existing tools like ProSight Lite were limited by platform dependency.
- There is a need for accessible, web-based proteoform analysis software.
Purpose of the Study:
- Introduce PSLite Online, a web-based application for top-down mass spectrometry data analysis.
- Expand upon the functionalities of ProSight Lite with enhanced usability and platform independence.
- Demonstrate the utility of PSLite Online for characterizing complex biological samples, such as antibody subunits with glycan modifications.
Main Methods:
- Developed a responsive, browser-based interface using modern web technologies.
- Integrated ProForma format for proteoform definition and modification.
- Implemented features for real-time fragmentation coverage visualization and mass deconvolution error correction.
- Enabled cloud-based session sharing via persistent URLs and SVG export.
Main Results:
- PSLite Online provides platform independence and enhanced usability compared to its predecessor.
- The application successfully facilitates targeted proteoform studies, including the characterization of antibody subunits with glycan modifications.
- Features like ProForma integration and cloud sharing improve data analysis and collaboration.
Conclusions:
- PSLite Online significantly broadens accessibility for proteomics researchers by removing installation barriers.
- The web-based application enables seamless collaboration across different devices and platforms.
- PSLite Online represents a modern, user-friendly solution for advanced proteoform analysis.
Related Concept Videos
00:59Mass Spectrometry: Alkene Fragmentation
3.7K
Alkenes lose one electron from the unsaturated π bond upon ionization and form stable molecular ions. Further fragmentation of alkenes occurs through three different reaction pathways. The most prominent fragmentation is the cleavage at the allylic position. The resultant allylic carbocation is resonance stabilized. In the mass spectra of terminal alkenes, this fragment appears at a mass-to-charge ratio of 41. In the internal alkenes, where there are two choices of allylic cleavage, the...
3.7K
00:55Mass Spectrometry: Amine Fragmentation
2.4K
Amines can be identified using mass spectroscopy based on their characteristic fragmentation patterns. The molecular ions of amines undergo fragmentation via ⍺-cleavage. The ⍺-cleavage of the carbon-carbon bonds in amines generates an alkyl radical and resonance-stabilized nitrogen-containing cation.
In amines, the number of nitrogen atoms affects the mass of the molecular ion, which is described by the nitrogen rule of mass spectrometry. This rule states that a compound containing a single...
In amines, the number of nitrogen atoms affects the mass of the molecular ion, which is described by the nitrogen rule of mass spectrometry. This rule states that a compound containing a single...
2.4K
01:05Mass Spectrometry: Cycloalkane Fragmentation
2.3K
In mass spectrometry, cycloalkanes exhibit distinct fragmentation patterns due to the inherent stability of their molecular ions compared to linear or branched alkanes. The ring structure of cycloalkanes provides additional stability to the molecular ions, often resulting in prominent ion peaks in the mass spectrum.
For example, cyclohexane molecular ions have a mass-to-charge ratio (m/z) of 84, which tends to produce a stronger signal than linear alkanes like hexane. This stability comes from...
For example, cyclohexane molecular ions have a mass-to-charge ratio (m/z) of 84, which tends to produce a stronger signal than linear alkanes like hexane. This stability comes from...
2.3K
00:54Mass Spectrometry: Cycloalkene Fragmentation
1.6K
The molecular ions of cycloalkenes undergo fragmentation via a retro-Diels–Alder reaction.
1.6K
00:53Mass Spectrometry: Alkyne Fragmentation
2.2K
The fragmentation of alkynes preferentially occurs at the carbon–carbon bond between the α and β carbon of the alkyne bond to generate a 3-propynyl cation (or propargyl cation). In terminal alkynes, there is the only type of fragmentation that yields the 3-propynyl cation. The unsubstituted 3-propynyl cation exhibits a peak at a mass-to-charge ratio of 39. In internal alkynes, the 3-propynyl cation is substituted. For example, 2-pentyne fragments into methyl-substituted 3-propynyl cation,...
2.2K
01:03Mass Spectrometry: Alcohol Fragmentation
4.6K
Alcohols (R-OH) ionize to lose one non-bonded electron from the oxygen atom, forming molecular ions. Due to their tendency to fragment rapidly, the intensity of the molecular ion peak in the mass spectrum is weak or sometimes absent. The fragmentation patterns for alcohols occur in two ways, i.e. ⍺-cleavage and dehydration. During ⍺-cleavage, the bond at the ⍺-position adjacent to the hydroxyl group cleaves to give a resonance-stabilized cation and a radical. However, intramolecular...
4.6K