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Updated: Feb 14, 2026

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
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Induced opening of conformational switch I in GTP-bound Ran GTPase

Janka Czigleczki1, Balint Dudas2, Erika Balog1

  • 1Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.

Protein Science : a Publication of the Protein Society
|February 13, 2026
PubMed
Summary

Ran GTPase regulates cell transport by switching between active and inactive states. This study reveals a new mechanism where disrupting key interactions forces the active RanGTP into an inactive state, offering therapeutic insights.

Keywords:
Ranconformational switchmolecular dynamicssmall GTPaseswitch I

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