Jatropadanes A-Q, lathyrane diterpenoids with lysosomal biogenesis potential from Jatropha podagrica Hook

Seventeen undescribed lathyrane-diterpenoids jatropadanes A-Q (1-17) were isolated from Jatropha podagrica Hook. Their structures were elucidated via spectroscopic data, single-crystal X-ray diffraction analysis, ECD and NMR calculations, Mo₂(OCOCH₃)₄ and Rh₂(OCOCF₃)₄ induced ECD experiments. Jatropodane A (1) formed a 5/5/10/3 ring system, with a new oxygen bridge between C-14 and C-5. Jatropadane B (2) promoted lysosomal biogenesis through the activation of the transcription factors TFEB/TFE3, whereas its inhibitory effects on the proliferation of HepG2 cells were attenuated by the autophagy inhibitor or the knockdown of TFEB and TFE3, suggesting that lysosomal biogenesis and autophagy induced by jatropadane B (2) were favorable programmed death of tumor cell lines.