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Challenges and opportunities in cell-free systems
Hue Vu Thi1, Yen-Vy Nguyen Thi1, Minh Chau Nguyen2
1The Interdisciplinary Research Group on Biomedicine and Health, International School, Vietnam National University, Hanoi, Vietnam; Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam.
Abstract:
Cell-free systems (CFS) are in vitro technologies (outside living cells) that use cellular components to reproduce cellular processes outside living organisms, such as protein synthesis, gene expression, and metabolic reactions. Nowadays, CFS has been improved over time and is considered one of the most useful tools for exploring and illustrating the fundamental principles of biological systems. In the medical field, CFS is being widely applied in vaccine production, gene expression research and point-of-care diagnostics. However, despite its significant benefits, CFS still faces many technological challenges and scientific limitations. In addition, sustainable development goes hand in hand with the balance between innovation and ethical, environmental and social issues. However, in the context of promoting biological solutions, CFS also opens many new opportunities. Therefore, challenges and some issues related to CFS technologies have been comprehensively evaluated in this chapter. The opportunities of CFS technologies have also been discussed in this chapter to highlight the huge potential of this technology in the near future. The evidence presented can serve as a foundational reference for research, teaching, and strategic policy-making in the field of next-generation synthetic biology.
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