Assembling a chromosome-level genome for the Microtus fortis using PacBio HiFi and Hi-C technologies

Area of Science:

• Genomics
• Comparative Genomics
• Mammalian Genetics

Background:

• The reed vole (Microtus fortis) is a valuable rodent model for unique biological traits.
• Its natural resistance to Schistosoma japonicum is of particular interest.
• Genetic studies require a high-quality reference genome.

Purpose of the Study:

• To produce the first chromosome-level genome assembly for Microtus fortis.
• To facilitate genetic research into the species' unique phenotypes.
• To provide a foundational resource for comparative genomics.

Main Methods:

• PacBio HiFi long-read sequencing was employed for genome assembly.
• Hi-C data was utilized for chromosome-level scaffolding.
• BUSCO analysis was performed to assess genome completeness.

Main Results:

• A 2.29 Gb genome assembly with excellent continuity (contig N50 = 68.89 Mb; scaffold N50 = 91.23 Mb).
• 97.7% of the sequence anchored to 26 pseudomolecules, matching the karyotype.
• Annotation identified 23,678 protein-coding genes, with 97.5% functionally assigned.

Conclusions:

• The high-quality genome assembly is a significant resource for Microtus fortis research.
• It will enable exploration of genetic mechanisms behind innate immunity and disease resistance.
• This assembly will enhance understanding of rodent evolution through comparative genomics.