Macrophyte Extracts Promote the Growth of the Microbial Community Associated With Microcystis aeruginosa Alleviating...

Macrophytes such as Pistia stratiotes and Pontederia crassipes can release allelopathic compounds and reduce cyanobacteria biomass. Cyanobacterial cells interact with heterotrophic bacteria, which contribute to nutrient uptake and antioxidative responses, among other functions. However, the role of microbial communities in allelopathic interactions between macrophytes and cyanobacteria remains unexplored. We investigated how the bacterial community associated with Microcystis aeruginosa influences the effects of aqueous macrophyte extracts. Both extracts inhibited cyanobacterial growth and photosynthetic activity (99% for P. stratiotes and 55% for P. crassipes) while increasing bacterial abundance (threefold). The composition of the bacterial communities stimulated by extracts shifted: whereas original cultures were rich in Methyloversatilis and Rhodobacter, the P. stratiotes extract promoted the growth of Shinella, Flavobacterium, and Comamonadaceae, and the P. crassipes extract favored Enterobacterales. When these stimulated communities were reintroduced into M. aeruginosa cultures, allelopathic inhibition was reduced (40% for P. stratiotes and 12% for P. crassipes). We concluded that the growth of the associated microbiota attenuated the allelopathic effects, partially preserving cyanobacterial cells. Bacterial groups favored by the treatments may participate in allelochemical degradation and antioxidant protection or activate other types of metabolism beneficial to cyanobacteria, mitigating the harmful effects of the extracts. These results highlight the importance of considering the role of microbial communities in cyanobacterial allelopathic interactions.