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Updated: Feb 16, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Real-time recombinase polymerase amplification (real-time RPA) detection of Staphylococcus aureus in foods
Qinglong Wang1, Liping Song1, Long Li2
1Beijing Food Inspection and Research Institute, Beijing Food Safety Monitoring and Risk Assessment Center, Beijing, China; Laboratory of Key Technologies of Major Comprehensive Guarantee of Food Safety for State Market Regulation, Beijing, China.
Abstract:
A rapid real-time recombinase polymerase amplification (real-time RPA) assay was developed and validated for the detection of Staphylococcus aureus (S. aureus). The assay achieved target-specific amplification within 20 min at 39 °C and a 50% limit of detection (LOD₅₀) of 298 CFU/mL. Method performance followed GB4789.45 and was evaluated using artificially contaminated samples from five food categories (frozen beverages, dairy, meat, cereals, and soy products). After a 6 h enrichment at 36 °C, matrix-wide relative limits of detection (RLOD) were < 2.5 across all matrices, meeting the acceptance criteria of GB 4789.45. Equivalence to the reference culture method was further assessed on naturally contaminated retail bulk pork, where chi-square testing showed no significant difference between real-time RPA and GB 4789.10 (p > 0.05). Collectively, these results demonstrate that the assay delivers same-day, post-enrichment detection with diagnostic outcomes comparable to culture, supporting its use as a rapid and reliable alternative to GB 4789.10 for routine monitoring of S. aureus in foods.
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