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Lectin-based detection and expression profiling of native glycoRNAs.

Yong Li1,2, Yisong Qian3, Xiang Li1

  • 1Department of Biomedical Science, School of Medicine, University of Missouri Kansas City, Kansas City, MO, 64108, USA.

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|February 14, 2026
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Summary
This summary is machine-generated.

Researchers developed a simple lectin hybridization method to detect glycosylated RNAs (glycoRNAs). This technique offers high sensitivity and broad applicability for studying glycoRNA expression in various samples, including biofluids.

Keywords:
DetectionExpression profilingGlycoRNAsLectinrPAL

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Glycobiology

Background:

  • Current methods for detecting glycosylated RNAs (glycoRNAs) like metabolic labeling and RNA-optimized periodate oxidation and aldehyde labeling (rPAL) have limitations.
  • There is a need for simpler, more rapid, and broadly applicable methods for glycoRNA detection.

Purpose of the Study:

  • To develop and validate a straightforward method for detecting native glycoRNAs using direct lectin hybridization.
  • To profile glycoRNA expression in various biological samples and conditions.

Main Methods:

  • Total RNA isolation followed by Northern blotting.
  • Direct lectin hybridization for glycoRNA detection.
  • Comparison with established glycoRNA detection methods.

Main Results:

  • The direct lectin hybridization method is highly sensitive, simple, and broadly applicable.
  • GlycoRNA expression was profiled in human and murine tissues, cell lines, and biofluids (plasma, urine, amniotic fluid).
  • Differences in glycoRNA expression were observed under physiological and pathological conditions.

Conclusions:

  • Direct lectin hybridization provides a reliable and reproducible alternative for studying glycoRNA biology.
  • The method enables the detection of free glycoRNAs in human biofluids for the first time.
  • This approach may offer utility for future clinical diagnostics.