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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Related Experiment Video

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Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
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Enhancing mouse fundus imaging with OCT embedding medium: Prolonging imaging duration and improving image quality.

Zilong Zhang1, Haolin Wang2, Shengliu Pan3

  • 1The Key Laboratory for Human Disease Gene Study of Sichuan Province and Department of Laboratory Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China; School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Experimental Eye Research
|February 15, 2026
PubMed
Summary

This study introduces an OCT embedding medium to improve mouse fundus imaging, reducing artifacts and dehydration for better visualization of retinal blood vessels in models like age-related macular degeneration.

Keywords:
Age-related macular degeneration (AMD)Mouse fundus imagingOCT embedding mediumRetinal blood vessels

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Area of Science:

  • Ophthalmology and Vision Science
  • Biomedical Imaging
  • Animal Models in Research

Background:

  • Fundus diseases are a major cause of vision loss, necessitating advanced research models.
  • Existing mouse fundus imaging methods face challenges like corneal whitening and motion artifacts, limiting research precision.
  • Mice are vital animal models for studying ophthalmic conditions and developing treatments.

Purpose of the Study:

  • To develop an innovative method for enhancing the quality and duration of mouse fundus imaging.
  • To overcome limitations of current techniques, particularly for prolonged, high-precision imaging in research settings.
  • To improve visualization of retinal structures, especially in disease models like age-related macular degeneration (AMD).

Main Methods:

  • Development and application of a novel Optical Coherence Tomography (OCT) embedding medium on the mouse cornea.
  • Utilizing the medium to improve eye-OCT lens alignment and reduce optical aberrations.
  • Evaluating the impact on imaging quality, corneal hydration, and artifact reduction during prolonged imaging sessions.

Main Results:

  • The OCT embedding medium significantly reduced stray light (flare) and motion artifacts during mouse fundus imaging.
  • Corneal dehydration was slowed, allowing for extended imaging periods.
  • Retinal blood vessel visualization was markedly improved, particularly in age-related macular degeneration (AMD) mouse models.

Conclusions:

  • The proposed OCT embedding medium offers a significant technical advancement for high-quality mouse fundus imaging.
  • This method enhances imaging precision and duration, crucial for studying ophthalmic diseases in animal models.
  • The technique shows promise for improving research outcomes and potential future clinical applications in ophthalmology.