Highly Sensitive Rhodol-Based Fluorescent Probe for Detecting Hydrogen Peroxide in Cells and Zebrafish

Area of Science:

• Biochemistry
• Chemical Biology
• Molecular Biology

Background:

• Hydrogen peroxide (H2O2) is a critical reactive oxygen species (ROS) involved in cellular signaling and oxidative stress.
• Dysregulation of H2O2 is linked to various human diseases, necessitating sensitive detection methods.

Purpose of the Study:

• To develop and validate a novel fluorescent probe for the sensitive and selective detection of H2O2.
• To demonstrate the probe's utility in monitoring endogenous and exogenous H2O2 in biological systems.

Main Methods:

• Synthesis and characterization of a new fluorescent probe.
• Evaluation of probe selectivity and sensitivity towards H2O2.
• Application of the probe for H2O2 detection in living cells and zebrafish models.

Main Results:

• The fluorescent probe exhibited high selectivity and sensitivity for H2O2 detection, with a low detection limit of 142.18 nM.
• The probe was successfully employed to visualize and quantify H2O2 levels in living cells.
• In vivo studies demonstrated the probe's effectiveness in detecting H2O2 in zebrafish.

Conclusions:

• The developed fluorescent probe is a valuable tool for sensitive and selective H2O2 detection.
• This probe facilitates the study of H2O2's physiological and pathological roles in complex biological systems.
• The findings open new avenues for investigating oxidative stress-related diseases.