Highly Sensitive Rhodol-Based Fluorescent Probe for Detecting Hydrogen Peroxide in Cells and Zebrafish

As a critical reactive oxygen species (ROS), hydrogen peroxide (H₂O₂) plays a significant role in regulating oxidative stress, which closely relates to various human diseases. Therefore, it is of great significance to sensitively detect H₂O₂ and elucidate its physiological function. Herein, a fluorescent probe was present for detecting H₂O₂ in living cells and zebrafish. It exhibited highly selective and sensitive responses in the presence of H₂O₂ with a detection limit of 142.18 nM. The probe was successfully applied to detect endogenous and exogenous H₂O₂ in living cells and zebrafish. All the results in this work demonstrated that the probe was a powerful tool for detecting H₂O₂ and exploring the roles of H₂O₂ in various physiological and pathological processes.