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Spatially selective multiphoton photothermolysis guided by reflectance confocal microscopy for precise vessel closure
Liwei Jiang1,2, Zhenguo Wu1,2, Jianhua Zhao1,2
1Basic and Translation Research Department, BC Cancer Research Institute, Vancouver, Canada.
Abstract:
Corneal neovascularization (CNV) is characterized by abnormal vessel growth into the cornea, often impairing vision. Conventional laser therapies for CNV lack precision and risk collateral damage. We developed a multiphoton photothermolysis (MPP) approach using femtosecond (fs) laser-based two-photon absorption guided by real-time reflectance confocal microscopy. Two MPP laser treatment configurations were implemented: dual lasers for imaging (785 nm CW) and treatment (830 nm fs), and a single fs laser for both functions. We demonstrated that in the mouse eye limbus, MPP achieved selective vessel closure without collateral tissue damage. Results also indicate therapeutic effects are mediated by two- rather than one-photon absorption, highlighting MPP's potential as a precise and safe CNV treatment.
Insights
Multiphoton photothermolysis (MPP) offers a precise method for treating corneal neovascularization (CNV). This advanced laser technique selectively closes abnormal blood vessels in the eye without causing collateral damage.
Area of Science:
- Ophthalmology
- Biomedical Engineering
- Laser Physics
Background:
- Corneal neovascularization (CNV) involves abnormal blood vessel growth in the cornea, leading to vision impairment.
- Current laser therapies for CNV often lack precision and can cause unintended damage to surrounding tissues.
Purpose of the Study:
- To develop and evaluate a multiphoton photothermolysis (MPP) approach for precise treatment of CNV.
- To investigate the efficacy and safety of MPP in selectively targeting and closing neovascular vessels in the cornea.
Main Methods:
- Developed an MPP system utilizing femtosecond (fs) laser-based two-photon absorption.
- Integrated real-time reflectance confocal microscopy for precise guidance.
- Implemented two configurations: dual lasers for imaging and treatment, and a single fs laser for both functions.
- Tested the technique in the mouse eye limbus model.
Main Results:
- MPP successfully achieved selective closure of corneal blood vessels.
- No collateral tissue damage was observed during the treatment.
- Therapeutic effects were confirmed to be mediated by two-photon absorption, not one-photon absorption.
Conclusions:
- MPP is a highly precise and safe method for treating corneal neovascularization.
- The two-photon absorption mechanism enhances the selectivity and safety of the laser treatment.
- This technology holds significant potential for improving vision restoration in patients with CNV.
