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Glutathione analysis and quantification in Korean supplements: method development and validation.

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Summary

A new liquid chromatography-ultraviolet method accurately analyzes reduced and oxidized glutathione in supplements. This quality control tool revealed significant variations in glutathione content across various products, aiding label accuracy verification.

Keywords:
Glutathione supplementLC–UVMethod validationOxidized glutathioneReduced glutathione

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Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Glutathione is a crucial endogenous antioxidant vital for cellular health.
  • Accurate quantification of glutathione in dietary supplements is essential for consumer safety and product efficacy.
  • Existing analytical methods often require derivatization, adding complexity and time.

Purpose of the Study:

  • To develop and validate a rapid, accurate, and derivatization-free liquid chromatography-ultraviolet (LC-UV) method for analyzing reduced and oxidized glutathione.
  • To assess the quality and label accuracy of commercially available glutathione supplements.

Main Methods:

  • Establishment and validation of an LC-UV detection method for reduced and oxidized glutathione.
  • Method validation included linearity (R² ≥ 0.999), accuracy (92.18-102.85% recovery), and precision (RSD ≤ 1.91%).
  • Application of the validated method to 60 diverse glutathione supplements (films, tablets, granules, powders) from the Korean market.

Main Results:

  • The developed LC-UV method demonstrated excellent linearity, accuracy, and precision.
  • Significant variations in actual glutathione content were found among different supplement products and formulations.
  • Discrepancies between measured and labeled glutathione content were identified in several samples, highlighting potential quality control issues.

Conclusions:

  • The developed LC-UV method provides a reliable and efficient tool for the quality control of glutathione supplements.
  • The findings underscore the importance of rigorous analytical testing to ensure the accuracy of product labeling and consumer safety.
  • This method is suitable for routine quality assurance and verification of label claims in the glutathione supplement industry.