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Bioaccessibility, composition, and anti-inflammtory activity of bound polyphenols from burdock (Arctium lappa L.)
Jue Cui1, Yu He1, Nannan Zhao1
1School of Food and Biology Engineering, Xuzhou University of Technology, Xuzhou, 221018 People's Republic of China.
Abstract:
Burdock (Arctium lappa L.), a pharmaco-culinary plant, is characterized by its high dietary fiber content. In this study, the bioaccessibility, polyphenol composition, and anti-inflammatory activity of bound polyphenols from burdock root dietary fiber (BRDF-BP) were investigated. In vitro digestion and colon fermentation results showed that BRDF-BP was mainly released from BRDF during the colon fermentation stage. BRDF-BP was characterized by HPLC-ESI-QTOF-MS/MS, revealing 23 polyphenols including four phenolic acids, one stilbene, and 18 flavonoids. BRDF-BP significantly ameliorated the LPS-induced inflammatory response in RAW264.7 cells by reducing ROS generation, inhibiting the secretion of inflammatory factors, and increasing antioxidant enzyme activities. Transcriptome results showed that BRDF-BP treatment significantly downregulated five inflammatory response-related signaling pathways, including NOD-like receptors, IL-17, and JAK-STAT. Network topology analysis revealed that Stat1 is a hub gene for BRDF-BP, which exerts anti-inflammatory effects. In conclusion, this study illustrates that BRDF-BP can be liberated during colonic fermentation and exhibits anti-inflammatory properties.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10068-025-02065-w.
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