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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: Jun 14, 2026

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Development of an immobilized system for RNA modification analysis.

Zhiyan Li1,2, Luyao Yu1,2, Xingyu Liu3

  • 1NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.

Molecular Omics
|February 16, 2026
PubMed
Summary
This summary is machine-generated.

This study developed a streamlined method for RNA modification analysis by immobilizing enzymes on beads. This technique efficiently converts RNA to ribonucleosides for mass spectrometry, aiding cancer research.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • RNA modifications are vital for cellular processes.
  • Current ribonucleoside sample preparation methods are limited to solution phase.
  • Need for efficient RNA modification analysis techniques.

Purpose of the Study:

  • To develop a streamlined system for RNA modification analysis.
  • To immobilize RNases for efficient RNA digestion.
  • To enable direct downstream mass spectrometry analysis.

Main Methods:

  • Utilized click chemistry (methyltetrazine and trans-cyclooctene) to immobilize RNases (nuclease P1, phosphodiesterase I, shrimp alkaline phosphatase) on agarose beads.
  • Developed a microspin tube modified with porous graphitic carbon for direct MS analysis.
  • Applied the system to monitor RNA modification dynamics during TGF-β-induced epithelial-mesenchymal transition in lung cancer cells.

Main Results:

  • Achieved complete RNA to ribonucleoside conversion within 30 minutes.
  • Demonstrated a streamlined workflow integrating immobilized RNases with MS analysis.
  • Observed significant changes in RNA modifications (m6A, m5U) during epithelial-mesenchymal transition in lung cancer cells.

Conclusions:

  • The developed method is efficient and robust for RNA modification analysis.
  • This approach supports the development of automated, high-throughput workflows.
  • RNA modification dynamics are crucial in tumor metastasis, as evidenced by changes during TGF-β-induced EMT.