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Mitigating sepsis-induced vascular endothelial dysfunction through Niban phosphorylation.

Brandon Baer1, Madeleine Morelli2, Brian Wadzinski3

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Reduced Niban phosphorylation contributes to sepsis-induced endothelial dysfunction by increasing endoplasmic reticulum (ER) stress. Treatment with NiPp, a Niban mimetic, restored vascular function in septic aortas.

Keywords:
Niban phosphorylation (NiPp)cytokinesendothelial functioninfectionoxidative stresssepsisvascular endotheliumvasoplegia

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Area of Science:

  • Vascular Biology
  • Sepsis Pathophysiology
  • Endothelial Function

Background:

  • Endothelial dysfunction is central to sepsis, driven by inflammation, oxidative stress, and ER stress, leading to organ failure.
  • Niban, a stress response protein, is crucial for endothelial homeostasis, with phosphorylation mitigating ER stress.
  • This study investigates the role of reduced Niban phosphorylation in sepsis-induced aortic dysfunction.

Purpose of the Study:

  • To determine if reduced Niban phosphorylation drives sepsis-induced endothelial dysfunction in the aorta.
  • To investigate if NiPp, a phosphopeptide mimetic of phosphorylated Niban, can restore vascular function.
  • To elucidate the mechanistic link between sepsis, Niban phosphorylation, and ER stress.

Main Methods:

  • Polymicrobial sepsis induced in rats using a cecal slurry model.
  • Analysis of ER stress markers and Niban phosphorylation via Western blot.
  • Assessment of aortic vascular reactivity and ex vivo NiPp treatment efficacy.

Main Results:

  • Septic aortas showed decreased Niban and eNOS phosphorylation, and increased GRP78.
  • Ex vivo NiPp treatment ameliorated endothelial dysfunction in septic aortas.
  • NiPp improved endothelium-dependent relaxation in aortic rings exposed to ER stressors or sepsis mediators.

Conclusions:

  • Reduced Niban phosphorylation is a key factor in sepsis-related vascular endothelial dysfunction.
  • A mechanistic link exists between sepsis, diminished Niban phosphorylation, and elevated ER stress.
  • NiPp shows therapeutic potential for restoring endothelial function during sepsis.