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Programmable DNA Framework-Based Logic-Gated Dual-Distance Regulation for Photoelectrochemical Multiplex and
Guixiang Chen1, Xinyi Liu1, Xingyu Liu1
1Hubei Key Laboratory of Pollutant Analysis and Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002, P. R. China.
Analytical Chemistry
|February 16, 2026
Summary
A new photoelectrochemical (PEC) sensor uses DNA frameworks for multiplex virus detection in water. This advanced sensor overcomes signal interference and low intensity, improving water safety monitoring.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- Multiplex virus detection in aquatic environments is crucial for water safety.
- Photoelectrochemical (PEC) sensors face challenges like charge recombination and signal cross-interference, limiting their use in environmental analysis.
- Existing methods struggle with sensitivity and specificity for detecting multiple viruses simultaneously.
Purpose of the Study:
- To develop a programmable, logic-gate-functionalized PEC sensor for sensitive and multiplexed virus detection in water.
- To address signal cross-interference and low signal intensity issues in PEC sensing.
- To enhance water safety monitoring through advanced analytical techniques.
Main Methods:
- Fabrication of a DNA-framework-mediated logic-gate-functionalized dual-distance-regulated PEC sensor.
- Programming DNA frameworks to construct logic operation units (OR, AND, OR-AND, AND-OR) for multiplex signal processing.
- Utilizing dual-distance regulation triggered by nucleic acid amplification to enhance signal response and mitigate charge recombination.
Main Results:
- The developed sensor successfully detected multiple viruses in water samples.
- Logic gate units effectively resolved signal cross-interference in multiplex detection.
- Dual-distance regulation significantly enhanced signal intensity, counteracting photogenerated charge recombination.
- Achieved a wide linear response range (0.001-500 nM) with a low detection limit of 7.7 fM for virus nucleic acids.
- Demonstrated robust analytical performance for coexisting virus nucleic acids in real water samples.
Conclusions:
- The programmable DNA-framework sensor offers a novel solution for multiplex virus detection in environmental monitoring.
- The integration of logic gates and dual-distance regulation overcomes key limitations of traditional PEC sensors.
- This approach holds significant potential for advancing single-interface PEC sensor applications in complex environmental analyses and ensuring water safety.
