Related Experiment Video
Updated: Feb 18, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Published on: March 20, 2016
The BBX6-2-NF-YC4-JAZ1 complex mediates SOC1 activation to control jasmonate-responsive flowering time in Brassica
Xianjun Feng1, Jingfei Li1, Jiaxing Ge1
1College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China.
Abstract:
Jasmonate (JA) accumulation and signaling play key roles in regulating plant growth and flower development. The optimal timing of flowering is critical for the quality and yield of Brassica juncea. B-box (BBX) factors play roles in plant floral transition and stress response processes. However, whether BBX6-2 responds to JA signaling to regulate flowering time in B. juncea remains unclear. Here, we characterized the biological function of BjuBBX6-2 in response to JA in regulating the flowering time of B. juncea. Subcellular localization and transcriptional activation activity assays showed that BjuBBX6-2 localizes in the nucleus and exhibits transcriptional activation activity. Spraying BjuBBX6-2-overexpressing or BjuBBX6-2-silenced B. juncea plants with 50 μmol/L methyl JA significantly accelerated or delayed their flowering time, respectively. We demonstrated that BjuBBX6-2 interacts with the flowering factor Nuclear Factor Y, Subunit C4 (BjuNF-YC4), which interacts with Nuclear Factor Y, Subunit B2/3 (BjuNF-YB2/3). The BjuBBX6-2-BjuNF-YC4-BjuNF-YB2/3 multiple-protein complex bound to the promoter of the downstream flowering integrator gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (BjuSOC1) and promoted its expression. JASMONATE ZIM-DOMAIN 1 (BjuJAZ1), a key factor in the JA signaling pathway, interacted with BjuBBX6-2 to inhibit the activation of BjuSOC1 by BjuBBX6-2. In summary, BjuBBX6-2 cooperates with BjuNF-YC4 and BjuJAZ1 in response to JA signaling to participate in the flowering regulation of B. juncea. These findings highlight a previously uncharacterized mechanism of JA signaling-mediated flowering time regulation via interactions between BjuBBX6-2 and the integrator gene BjuSOC1, providing prospects for breeding enhanced B. juncea cultivars.
More Related Videos
07:55Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Published on: November 20, 2017
04:11Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
Related Concept Videos
Cell Signaling in Plants
Plant Hormones
Gene Regulation During Sporulation
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Cell Adhesion in Plants
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...