Hirsutanines H-O, monoterpene indole alkaloids from Uncaria hirsuta with neuroprotective effects
Nan An1, Lei-Ming Wu1, Shu-Qi Wu1
1School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
Abstract:
Hirsutanines H-O (1-8), eight previously undescribed monoterpene indole alkaloids, along with five known analogues (9-13), were isolated from the stems and leaves of Uncaria hirsuta. Compounds 1-4 represent a rare subclass of 3-oxo-3,7-seco-oxindole alkaloids. The structures of 1-13 were elucidated by spectroscopic, computational, and chemical means. All the isolates were evaluated for their neuroprotective effects against sodium nitroprusside-induced damage in PC-12 cells. Among them, compounds 1, 4, and 9 exhibited pronounced activity, being comparable to that of the positive control, edaravone. Mechanistic studies revealed that 1, the most active compound, exerted its neuroprotective effect via attenuating oxidative stress.
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